File:A-Novel-Chemotaxis-Assay-in-3-D-Collagen-Gels-by-Time-Lapse-Microscopy-pone.0052251.s001.ogv
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A-Novel-Chemotaxis-Assay-in-3-D-Collagen-Gels-by-Time-Lapse-Microscopy-pone.0052251.s001.ogv (Ogg Theora video file, length 12 s, 336 × 238 pixels, 85 kbps)
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English: Directional migration of freshly isolated human neutrophils in 3D collagen matrix towards Il-8. Images were taken at 10× magnification, and correspond to the Z projection of 5 layers reported at a reduced time and spatial resolution with about one frame every two minutes, in order to minimize the file dimension. The first 30 minutes, where each frame has a white overlay showing the progressive time and a dimension bar, are representative of the unstimulated condition where a limited fraction of cells move randomly. The IL-8 solution was added to the reservoir at t = 30 min. In the second part of the movie where the overlay is in black, cells are under the effect of an IL-8 concentration gradient. An increase in cell motility, that has been quantified in terms of average velocity and fraction of motile cells, and a preferential movement toward the source of the chemoattractant, i.e. towards the bottom part of the image, can be clearly noticed.
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Source | Movie S1 from Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S (2012). "A Novel Chemotaxis Assay in 3-D Collagen Gels by Time-Lapse Microscopy". PLOS ONE. DOI:10.1371/journal.pone.0052251. PMID 23284956. PMC: 3526591. | |||||||
Author | Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S | |||||||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:00, 5 January 2013 | 12 s, 336 × 238 (121 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Update transcode statusFormat | Bitrate | Download | Status | Encode time |
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WebM 360P | 162 kbps | Download file | Completed 13:06, 5 January 2013 | 2.0 s |
Ogg 160P | 73 kbps | Download file | Completed 06:49, 21 February 2014 | 1.0 s |